Below, you’ll learn what clinical researchers discovered when they treated one of the world’s most difficult-to-kill pathogens – Bacillus subtilis – with silver nanoparticles.

Once again, science proves it’s the silver ion that’s responsible for silver’s astonishing effectiveness against some of the most hardy and difficult-to-eradicate pathogens on the face of the earth.

Below, you’ll learn what clinical researchers discovered when they treated one of the world’s most difficult-to-kill pathogens – Bacillus subtilis – with silver nanoparticles.

Here’s a hint: Researchers discovered that it wasn’t the metallic silver nanoparticles that killed the pathogen. It was the silver ions released by the metallic silver nanoparticles that caused the fatal damage.

This proves once again that ionic silver is the active, infection-fighting form of colloidal silver, and that all other forms of silver are effective only to the extent they release silver ions in the presence of pathogens.

Study about Bacillus subtilis and Nano silver 

Bacillus subtilis – google image 

In a clinical study with the lengthy title “The Antimicrobial Properties of Silver Nanoparticles in Bacillus subtilis Are Mediated by Released Ag+ Ions,” researchers discovered that silver nanoparticles exhibit profound antimicrobial action against the Bacillus subtilis bacterium – one of the world’s most difficult-to-kill microbes.

But here’s the clincher: That effectiveness was due largely to the release of silver ions from the metallic silver nanoparticles used in the experiment, and not due to the metallic silver nanoparticles themselves.

Although Bacillus subtilis is a relatively benign soil bacteria, scientists know that it’s extremely difficult to kill due to its ability to produce endospores – a kind of “armor plating” which makes the microbe virtually impervious to a variety of disinfectants and toxic chemicals including antibiotic drugs.

Thanks to this “armor plating,” the pathogen is also virtually immune to temperature variations, desiccation, ultra-violet light, and even to starvation since it can go dormant for months or even years at a time, hiding within its tough endospore shell whenever it feels threatened by antibiotic drugs or other substances.

But the researchers behind the above-mentioned study found that silver ions busted right through the tough outer shell of Bacillus subtilus, just as previous researchers have discovered that silver ions bust through the bacterial biofilms used by colonies of drug-resistant super-pathogens to protect themselves against antibiotic drugs.

Similar to Anthrax

Bacillus subtilis

Bacillus anthracis – google image 

Because of its unusual characteristics, Bacillus subtilis is often used in clinical testing as a substitute for its first-cousin Bacillus anthracis – yes, the deadly anthrax pathogen, which has similar qualities that make it just as difficult to eradicate.

Indeed, for decades Bacillus subtilis has been used by the U.S. Army as a “simulant” in germ warfare studies of the anthrax pathogen. When the military wants to know if a substance can kill anthrax, they first test that substance againstBacillus subtilis. If the substance can kill Bacillus subtilis, then the military researchers can be relatively certain it can kill anthrax as well.

In the above-mentioned clinical study, researchers treated Bacillus subtilis cultures with 0-50 ppm concentrations of silver nanoparticles, and found that while a concentration of only 5 ppm inhibited bacterial growth for 12 hours, concentrations of 10 ppm and higher were lethal to the otherwise difficult-to-kill bacterium.

The researchers found that reactive oxygen species triggered by the release of positively charged silver ions from the silver nanoparticles contributed to the breakdown of the tough “armor plated” cell membrane of the microbe, and that the ionic silver then penetrated the cell membrane and disrupted the integrity of the microbe’s chromosomal DNA causing it to die.

The researchers concluded:

“To the best of our understanding, this is the first study to directly analyze silver particles present within bacterial cells treated with silver nanoparticles, and the results indicate that positively charged silver ions are primarily responsible for silver nanoparticle microbial toxicity…

…our results support the theory that silver nanoparticles exert microbial toxicity through the release of positively charged silver ions that subsequently penetrate into bacterial cells.”

Once again, this is very important to understand:

The researchers treated the microbes with relatively low levels of metallic silver particles (i.e., silver nanoparticles). But they discovered that the metallic silver particles didn’t kill the hardy microbes.

Instead, the silver ions being released by the metallic silver particles did the killing, first, by releasing reactive oxygen species (much like hydrogen peroxide) that essentially “softened up” the tough outer microbial shell of the pathogen, and second, by penetrating that damaged shell wall, entering the microbe, and binding to its DNA.

This proves, once again, that the silver ion (i.e., ionic silver) is the active, infection-fighting form of silver, and that metallic silver (i.e., silver nanoparticles) is only effective to the extent it releases silver ions in the presence of the pathogens.

This is why smart colloidal silver users always choose the superior ionic form of colloidal silver over the metallic silver nanoparticle form. They know the metallic form must first be converted into the ionic form inside the human body in order to work – a process which is both slow and inefficient compared to utilizing pure ionic colloidal silver instead.

Indeed, the human body is able to absorb 90-100% of the tiny silver micro-particles, sending them throughout the body to kill pathogens, and then afterwards excreting them with ease, thanks to their small size.


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